RNA Quality Evaluation Service Details
RNA Quality Evaluation
As part of this service, Users will obtain a report with size fractionation of the nucleic acids in two views (electrophoretogram and gel image) and an RQI (RNA Quality Indicator) score, where applicable.
Turn around time is usually 48 hours. In some cases, it may take up to 72 hours to complete a run.
The Facility will provide a detailed report of the sample run, which will be e-mailed to the User.
RNA Qubit Quantitation
As part of this service, Users will obtain a duplicate Qubit read along with the average [ng/ul] for each sample submitted. We offer the Qubit broad range (BR) assay, 20-1000ng range.
Quantitative Range: 25-500ng/ul
Sizing Range: 100-6000nt
Volume Used: 1ul
High Sensitivity R6K
Quantitative Range: 100-10,000pg/ul
Volume Used: 2ul
Users need to register their samples through iLab. Please go to the Customer Portal and make your request.
Please bring your samples within quantitative range of the assay: we will NOT dilute your sample. Only 1 ul of sample is required for the the standard assay, and 2ul for the high sensitivity assay. Sample permitting, we ask you supply more in case we need to rerun the sample if needed.
8 or less samples can be brought in tubes or plates. 9 or more samples must be brought in plates in column format.
Your RNA samples will be subjected to heat treatment for this protocol, degrading them in the process. Samples will be disposed after processing. Please DO NOT provide us with all your sample, only an aliquot.