RNA Quality Evaluation Service Details

Service Features

RNA Quality Evaluation

As part of this service, Users will obtain a report with size fractionation of the nucleic acids in two views (electrophoretogram and gel image) and an RQI (RNA Quality Indicator) score, where applicable.

Turn-Around Time:

Turn around time is usually 48 hours. In some cases, it may take up to 72 hours to complete a run.

Data Retrieval:

The Facility will provide a detailed report of the sample run, which will be e-mailed to the User.

RNA Qubit Quantitation

As part of this service, Users will obtain a duplicate Qubit read along with the average [ng/ul] for each sample submitted. We offer the Qubit broad range (BR) assay, 20-1000ng range.

TAPE SPECIFICATIONS

Standard RNA    

  •     Sensitivity: 5ng/ul

  •     Quantitative Range: 25-500ng/ul

  •     Sizing Range: 100-6000nt

  •     Volume Used: 1ul

 

High Sensitivity R6K        

  •     Sensitivity: 100pg/ul

  •     Quantitative Range: 100-10,000pg/ul

  •     Volume Used: 2ul

User Instructions

Sample Registration:

Users need to register their samples through iLab. Please go to the Customer Portal and make your request.

Sample Submission:

Please bring your samples within quantitative range of the assay: we will NOT dilute your sample. Only 1 ul of sample is required for the the standard assay, and 2ul for the high sensitivity assay. Sample permitting, we ask you supply more in case we need to rerun the sample if needed.

8 or less samples can be brought in tubes or plates. 9 or more samples must be brought in plates in column format.

IMPORTANT

Your RNA samples will be subjected to heat treatment for this protocol, degrading them in the process. Samples will be disposed after processing. Please DO NOT provide us with all your sample, only an aliquot.

Pricing

 
 
 
2103 Cornell Rd.
WRB 3-401
Cleveland, OH 44106

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